Table 3.
Activity Table for the Purification of Strep2-PhaCRe4h.
| Purification Step | Volume (mL) | Protein (mg) | Activity (Ua/mg) | Total units | % Yield |
|---|---|---|---|---|---|
| Crude extractc | 5.0 | 140 | 3.4 | 470 | 100 |
| Soluble fraction | 4.3 | 120 | 2.8 | 340 | 72 |
| Insoluble fraction | 2.1 | 15 | 8.7 | 130 | 28 |
| Flow through | 7.2 | 89 | 1.2 | 110 | 22 |
| Washes | 60 | 28 | 1.1 | 31 | 6 |
| Pure sample | 0.52 | 0.94 | 36 | 34 | 7 |
a
One unit of enzyme activity is defined by one μmol of CoASH released per min.
c
Crude extract for Strep2-PhaCRe4h purification originated from 1 g of cells.