. Author manuscript; available in PMC: 2013 Mar 7.

Published in final edited form as: J Am Chem Soc. 2012 Feb 22;134(9):4229–4235. doi: 10.1021/ja210118w

Table 2.

Energetics of Domain Swapping and Protein Unfolding of WT CV-N and its Variants

	Kinetic parameters for domain swapping measured by NMR			Thermodynamic parameters for unfolding measured by DSC
	wt CV-N (M → D)	wt CV-N (D → M)	CV-N^P51G (D → M)	CV-N^P51G (M → U)	CV-N^P51G (D → U)	CV-N^ΔQ50 (D → U)
ΔH^‡ or ΔH kcal/mol	145 ± 22 (323.9-326.9K)	153 ± 15 (322.5-325.5K)	162 ± 32 (327.8-330.5K)	130 ± 1	171 ± 4	142 ± 1
ΔS^‡ cal/(mol·K)	363 ± 66 (323.9-326.9K)	391 ± 45 (322.5-325.5K)	410 ± 97 (327.8-330.5K)	-	-	-
ΔG^‡ kcal/mol	26.8 ± 0.1 (325.5K)	25.2 ± 0.1 (325.5K)	27.3 ± 0.1 (327.8K)	-	-	-
k₁ or k_-1 × 10^-6 s ^-1	6.6 ± 0.3 (325.5K)	82.0 ± 2.6 (325.5K)	4.3 ± 0.5 (327.8K)	-	-	-