Figure 7.

Effects of Src and ROS inhibitors on EC permeability and monolayer disruption induced by 100 μg/ml OxPAPC. HPAECs were pretreated with vehicle, PP2 (1 μM), or NAC (1 mM) for 30 minutes, followed by stimulation with 100 μg/ml OxPAPC. (A) Measurements of TER were monitored for 20 hours. The data shown here were pooled from three independent experiments, and are expressed as means ± SD. (B and C) Immunofluorescence staining for F-actin (B) or VE-cadherin (C) was performed after 15 minutes of OxPAPC stimulation. Arrows indicate areas of intercellular gaps. The results shown are representative of three independent experiments.