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. 2012 Mar 26;109(15):5651–5656. doi: 10.1073/pnas.1200318109

Fig. 3.

Fig. 3.

Defining the HCF107 binding site. (A) Diagram of the psbT–psbH intergenic region. These genes lie within the psbB-psbT-psbH-petB-petD transcription unit. The sequence shows the 5′ end of the RNAs that fail to accumulate in hcf107 mutants. The HCF107 binding site (as established through the assays shown in B) is underlined; dashes indicate nucleotides that likely contribute to binding affinity but that are not essential for binding. (B) Gel mobility shift assays used to define the HCF107 binding site. Binding reactions used rHCF107 (0, 50, 100, and 200 nM) under the same conditions as in Fig. 1B with 0.5 mg/mL heparin. The sequences of the RNAs are shown below. (C) Equilibrium Kd of rHCF107 for RNA 1 and RNA 10. Binding reactions were performed as in B. The raw data are shown in Fig. S3. (D) Multiple sequence alignment showing conservation of the HCF107 binding site among angiosperms. At, A. thaliana; Hv, Hordeum vulgare; Nt, Nicotiana tabacum; Os, Oryza sativa; Pa, Populus alba; Zm, Zea mays.