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. 2012 Mar 26;109(15):5862–5867. doi: 10.1073/pnas.1113819109

Fig. 1.

Fig. 1.

FEZ1 interacts with Munc18 and Stx. (A) Identification of FEZ1 as an interactor of Munc18 and Stx using a Y2H assay. Bait constructs containing fragments of Munc18 and Stx (without its transmembrane domain) were used to screen preys containing different FEZ1 fragments [amino acid residues (aa) given in parentheses]. (B and C) Validation of FEZ1 interactions by coimmunoprecipitation. HEK 293 cells expressing tagged FEZ1 with either Stx or Munc18 (black circles on top of the lanes) were immunoprecipitated (IP) using tag-specific antibodies (anti-GFP) and analyzed by immunoblotting (IB) for the presence of GFP-FEZ1, Myc-Stx, and FLAG-Munc18, respectively. FEZ1 interacts with both Munc18 and Stx. Input corresponds to 1% of the starting material used for immunoprecipitation. Molecular mass markers indicated are in kilodaltons.