Fig. 1.
Mycobacterial glycopeptidolipid (GPL) biosynthesis involves thioester reduction to alcohol: (A) Proposed mechanism of chain release during GPL biosynthesis. (B) HPLC analysis of RGPL assay with valeryl-L-Phe-L-Thr-L-Ala-L-Ala-CoA (valeryl-FTAA-CoA) and NADPH shows valeryl-FTA-Alaninol (peak II) as the major product along with valeryl-FTA-Alaninal (peak I) and valeryl-FTA-Alanine (peak III). Control reaction in the absence of protein did not show any product. (C) Tandem mass spectrometric analysis of valeryl-FTA-Alaninol peak at m/z [M-H]- = 477.3.