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. 2012 Mar 26;109(15):5681-5686. doi: 10.1073/pnas.1118680109

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Fig. 2. — FAAL10-nrp genetic cluster of Mtb: (A) Scheme showing the predicted function of FAAL10 and Rv0100 in the synthesis of a novel metabolite by this genetic cluster. (B) FAAL10 converts octanoic (C8) acid, decanoic (C10) acid, lauric (C12) acid and palmitic (C16) acid, but not hexanoic (C6) acid to corresponding acyladenylates. No acyl-CoA is formed when Coenzyme A (CoASH) is added to the reaction. Control reaction was set up using FACL19¹⁴ and lauric acid. (C) Coupled assays of FAAL10 with Rv0100, the left box shows proteins FAAL10 (approximately 60 kDa) and Rv0100 (approximately 10 kDa) used in the assay. The corresponding autoradiogram in the right box shows that in the presence of ATP and ¹⁴C lauric acid, the acyl chain gets transferred to the ppant arm of Rv0100 (shown with an arrow). (D) HPLC analysis of R_NRP assay with valeryl-FTAA-CoA and NADPH showed valeryl-FTA-Alaninol (peak II) as the major product along with valeryl-FTA-Alaninal (peak I) and valeryl-FTA-Alanine (peak III).