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. 2012 Mar 26;109(15):5675–5680. doi: 10.1073/pnas.1114905109

Fig. 5.

Fig. 5.

Knockdown of Ctr9 did not alter the H3R17me2a level at the ERE of pS2; however, H3K4me3 level was reduced. (A) ChIP assays showed the occupancies of Cdc73, Paf1, total RNAPII, and CTD S2-phosphorylation at distal and proximal promoter and encoding regions of the pS2 gene. (B and C) Quantitative PCR of ChIP products showed that knockdown Ctr9 did not affect H3R17me2a level at the ERE region of the pS2 gene (C); however, H3K4me3 level at the ERE was significantly decreased (B, *P < 0.02). (D) Ash2L is effectively knocked down after 6-d Dox treatment in MCF7-tet-on-shAsh2L cells. (E–G) ChIP quantitative PCR results showed knockdown of Ash2L decreased H3K4me3 level at the ERE of pS2 (E, *P < 0.002), but had negligible effect on H3R17me2a level (F) and recruitment of Cdc73 (G). Error bars represent SD (n = 3).