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. 2012 Mar 26;109(15):5785–5790. doi: 10.1073/pnas.1116158109

Fig. 1.

Fig. 1.

sod quintuple mutant worms have no SOD activity. (A) PCR genotyping reveals that sod-12345 worms have deletions in all five sod genes. (B) Quantitative real-time RT-PCR shows that sod-12345 worms have little or no sod mRNA expression. (C) Western blotting using polyclonal antibodies against SOD-1 and SOD-2 reveals that sod-12345 worms have no SOD-1 or SOD-2 protein expression. (D) sod-12345 worms have no detectable SOD activity. The generation of a bona fide sod quintuple mutant was thus confirmed by DNA, mRNA, protein, and activity. Error bars indicate SEM. M, sod-12345 worms.