Fig. 3.

Normal chemotaxis of EpCAM-deficient LC in collagen gels. (A) Representative tracks of single LC migrating toward CCL19 within type I collagen networks. Fifty LC of each genotype were tracked, and individual tracks are displayed in a 2D graphic. The intersection of horizontal and vertical lines represents the originating location for each cell at the beginning of the observation period. (B) Quantification of LC chemotaxis in vitro. Velocities of WT and EpCAM-deficient LC migrating toward CCL19 in collagen gels. Migration was measured over 3 h at 1-min intervals using 10× magnification (10 cells per field were tracked, five fields per group). Data depicted represent mean velocities ± SEM of three experiments. No TX, no treatment.