Fig. 6.

High-resolution confocal immunfluorescence of LC in situ reveals altered morphology in LC/EpCAM-cKO mice. (A) Epidermal sheets were prepared from LC/EpCAM-cKO and WT mice at steady state, stained with anti-MHC Class II mAb and anti-ZO-1 pAb, visualized using confocal microscopy, and are presented as 2D projections of z-volumes (WT, z = 35 μm; KO, z = 30 μm). (Scale bar: 15 μm.) (B) Confocal images of intraepidermal LC after incubation of skin explants with CCL21 (0.1 μg/mL) for 72 h and staining with anti-MHC Class II mAb. (Scale bar: 10 μm.) (C) Composite en face images and 90° rotation z-stacks of MHC Class II-expressing cells in epidermis at steady state (Scale bar: 10 μm.)