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NMR HSQC spectra of the different charge variants of S6. All spectra show wild-type like dispersion, suggesting that the supercharged S6+1-17 and the charge-depleted S6+1 maintain fixed, three-dimensional structures. Additional NMR evidence for conserved structure of S6+1-17 is presented in Fig. S2, whereas detailed structural analysis of S6+1 has so far been precluded by aggregation on longer timescales (cf. Fig. 5).
