Fig. 2.

Protein p6 colocalizes with TP at the cell nucleoid at middle post-infection times. (A) B. subtilis 110NA cells were grown at 37 °C in LB medium containing 5 mM MgSO₄. At an OD₆₀₀ of 0.45–0.5, the culture was infected with wild-type phage ϕ29 at a MOI of 5. Samples were harvested at the indicated times after infection and processed for IF microscopy using polyclonal antibodies against p6 and TP. (B) B. subtilis strain IH-19 (expressing protein p6 constitutively and TP under an inducible IPTG promoter) was grown at 37 °C in LB medium containing 5 mM MgSO₄. At an OD₆₀₀ of 0.4, 10 μM IPTG was added to induce expression of TP. Samples were harvested at 30 and 40 min post induction.