Skip to main content
. 2012 Mar 26;109(15):5744–5749. doi: 10.1073/pnas.1110627109

Fig. 4.

Fig. 4.

NF-κB regulates embryonic and prepubertal ductal development and is essential for Eda-induced accelerated branching morphogenesis. (A and B) Whole-mount stained fourth inguinal mammary glands of female IκBαΔN and littermate control mice at E18. (C) Quantification of epithelial branching, expressed as the average number of ductal tips ± SD per fourth mammary gland in control (n = 13) and IκBαΔN (n = 18) mice at E18. (D and E) Whole-mount stained fourth inguinal mammary glands of female IκBαΔN and their littermate control mice at 3 wk of age. (F) Average number of ductal tips ± SD per fourth mammary gland of 3-wk-old IκBαΔN mice (n = 18) and littermate controls (n = 13), of 3-wk-old mice normalized to weight (control, n = 13; IκBαΔN, n = 10), and of 3-wk-old IκBαΔN mice (n = 8) compared with weight-matched controls (n = 8). (G–N) Whole-mount stained fourth inguinal mammary glands of female control, K14-Eda, IκBαΔN, and K14-Eda;IκBαΔN compound mutants at E18 (G–J) and at 3 wk of age (K–N). n, number of glands analyzed. **P < 0.01; ***P < 0.001. (Scale bars: 500 μm.)