Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Nov 7;13(6):R112. doi: 10.1186/bcr3054

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright ©2011 Banerjee et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

TWIST1 is downstream of ARTEMIN (ARTN) stimulated Protein kinase B (AKT) activation in estrogen receptor (ER) negative mammary carcinoma (ER-MC). (a) Western blot analyses for ARTN, TWIST1, phospho AKT (pAKT-Ser473) and AKT in BT549 cells with forced expression of TWIST1 ± siARTN. β-ACTIN was used as loading control for cell lysates. The sizes of detected protein bands in kiloDalton (kDa) are shown on the right. (b) Colony formation in soft agar. (c) Cell growth (number) in 3D matrigel culture. (d) Cell migration, and (e) Cell invasion was determined for BT549-TWIST1 cells ± siARTN. siRNA control-treated vector (VEC) cells are presented as 100%. **, P < 0.01; ***, P < 0.001.