Table 2.

Estrogen-regulated miRNA profiles of ER+ breast cancer cell line MCF-7 using TaqMan Low-Density Array

Downregulated miRNAs			Upregulated miRNAs
miRNAs	Fold change	P-value	miRNAs	Fold change	P-value
miR-509	-119.389	<0.001	miR-189	5.024565	0.002
miR-376a	-102.459	<0.001	miR-326	5.52856	<0.001
miR-629	-91.9202	<0.001	miR-520c	5.619023	<0.001
miR-34b	-71.9166	<0.001	miR-520b	5.787208	<0.001
miR-519d	-63.3272	<0.001	miR-329	6.293726	<0.001
miR-33	-41.4439	<0.001	miR-17-3p	7.373741	<0.001
miR-515-5p	-40.7382	<0.001	miR-369-3p	8.591994	<0.001
miR-624	-38.3289	<0.001	miR-519c	8.613923	<0.001
miR-34c	-37.07	<0.001	miR-524	11.21323	<0.001
miR-551b	-33.1565	<0.001	miR-576	13.31873	<0.001
miR-564	-27.6564	<0.001	miR-653	13.9337	<0.001
miR-449	-25.5663	<0.001	miR-383	15.40733	0.004
miR-10b	-22.5561	<0.001	miR-627	15.45773	<0.001
miR-518e	-22.0561	<0.001	miR-105	16.18497	0.001
miR-139	-21.8713	<0.001	miR-616	20.96113	0.032
miR-520h	-18.821	<0.001	miR-422b	24.21192	0.018
miR-453	-17.2921	<0.001	miR-452	28.38138	<0.001
miR-526b	-10.5159	0.014	miR-380-5p	33.71417	0.007
miR-371	-9.59159	<0.001	miR-548b	42.83806	<0.001
miR-656	-8.11175	0.001	miR-521	44.27376	0.005
miR-124a	-6.73618	<0.001	miR-579	45.54916	<0.001
miR-518f	-5.80659	0.002	miR-142-5p	51.74748	<0.001
miR-135a	-5.40944	<0.001	miR-519e	53.65128	<0.001
miR-485-5p	-5.01188	0.003	miR-185	55.94657	<0.001
			miR-493	92.40058	<0.001
			miR-515-3p	97.85771	<0.001

ER = estrogen receptor. The expression profiles of miRNAs which have at least fivefold changes in expression. We used U6B expression as the internal control to normalize expression levels between the two groups. Experiments were repeated in triplicates. Relative expression = 2^^{-(with 10 nM estradiol/without estradiol)}.