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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Peptides. 2012 Feb 2;34(2):380–388. doi: 10.1016/j.peptides.2012.01.020

Figure 4. Ang-(1-7) reduces phospho-ERK1/ERK2.

Figure 4

Cardiac fibroblasts pre-treated with 100 nM Ang-(1-7) [A7] were stimulated with 10 nM ET-1 (Panel A) or 100 nM Ang II (Panel B) and phospho-ERK1/ERK2 were determined using a phospho-specific antibody. Representative blots show ET-1 or Ang II-stimulated phospho-ERK1/ERK2 and actin immunoreactivity in the presence or absence of Ang-(1-7). Graphs show percent control of ET-1 or Ang II phospho-ERK1 and ERK2. n = cells isolated from 2 to 4 litters of neonatal pups, in duplicate or triplicate. * p <0.05 compared with Ang II alone; **denotes p < 0.01 compared with ET-1 treatment alone.