Skip to main content
. 2001 May 8;98(10):5643–5648. doi: 10.1073/pnas.091584598

Figure 1.

Figure 1

Presentation of ES-cell generation and analysis of ES-cell clones. (A) Schematic representation of ES-targeting strategy. (B) Targeting construct. The targeting vector is designed to replace the exon of Flk1 that encodes the initiating methionine with a cassette containing int-3HA and the neomycin (neo) gene cassette. (C) Genotype analysis of ES clones by Southern blotting. The targeted locus places int-3HA under the gene-regulatory region of Flk1 and introduces a NotI (N) site at the junction of the int-3HA and neo genes. Genomic DNA was digested with NotI, and hybridization was done with an Flk1-specific probe. (D) Expression analysis for int-3HA transcripts in embryoid bodies (EBs) by RT-PCR. Analysis was conducted with control RNA from kidney, from parental line 1D5, and from a homologous recombinant line, 1A12.