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. Author manuscript; available in PMC: 2012 Apr 16.
Published in final edited form as: J Endocrinol. 2011 Jun 3;210(3):271–284. doi: 10.1530/JOE-11-0083

Figure 8.

Figure 8

NO and superoxide production by wild-type and the ZnS4 cluster mutants of eNOS. Recombinant wild-type eNOS and the ZnS4 cluster mutants (C94A, C99A, and C9499A) were incubated with L-arginine and the appropriate co-factors for 30 min at 37 °C and NO (A) and superoxide levels (B) determined as described (Grobe et al. 2006). There is a significant reduction in both NO (A) and superoxide (B) generation in the ZnS4 cluster mutants. Data are presented as mean±S.E.M.; n=3. *P<0·05 versus wild-type eNOS. †P<0·05 versus C94A mutant.