Table 3.

Production of nonprenylated and of prenylated phenazines in the heterologous expression strain Streptomyces coelicolor M512, carrying either the complete endophenazine gene cluster (ppzOS04) or clusters in which individual genes had been deleted. Data represent mean values ±SD.

integrated construct	description	nonprenylated phenazines [µmol/L] (phenazine-1-carboxylic acid and phenazine-1-carboxylic acid methyl ester)	prenylated phenazines [µmol/L] (endophenazine A, B and E)
ppzOS04	complete cluster	17.8 ± 4.0	200.8 ± 42.7
ppzOS21	Δ(orf1 − orf2)	6.7 ± 2.4	161.5 ± 35.9
ppzOS10	Δ(orf1 − orf4)	54.9 ± 4.2	23.3 ± 1.6
ppzOS22	Δ(orf1 − middle of orf4)	12.4 ± 1.9	112.8 ± 20.4
ppzOS23	ΔppzT	7.3 ± 1.6	103.9 ± 16.8
ppzOS24	Δ(ppzT + ppzU)	6.1 ± 1.7	2.4 ± 0.9
ppzOS25	ΔppzV	58.3 ± 21.0	<0.1
ppzOS26	ΔppzM	4.5 ± 0.5	165.5 ± 60
ppzOS11	Δ(ppzK − orf12)	1.1 ± 0.3	0.4 ± 0.2
ppzOS27	Δ(ppzZ1 − orf12)	17.5 ± 3.5	3.1 ± 1.07
ppzOS28	Δ(ppzR1 − orf12)	14.8 ± 5.2	31.4 ± 5.1