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. 2012 Feb 27;3(2):158–171. doi: 10.18632/oncotarget.442

Figure 1. Generation of PRL-3 specific chimeric mAb.

Figure 1

A. A schematic diagram outlining the major steps of PRL-3 chimeric mAb construction. B. PRL-3 chimeric mAb recognizes EGFP-PRL-3 overexpressed in DLD-1 human colorectal cancer cells by indirect immunofluorescence: a, distribution of EGFP-PRL-3 in fixed DLD-1 cells (green); b, PRL-3 chimeric antibody and anti-human IgG Texas-Red to reveal the binding to PRL-3 protein; c, merged image. Bar, 20 μm. C. Cell lysate derived from DLD-1 cells overexpressing EGFP-PRL-3, and lysates derived from CHO stable cell lines overexpressing myc-PRL-3, myc-PRL-1, and myc-PRL-2 were analyzed by western blotting. PRL-3 chimeric antibody specifically recognized EGFP-PRL-3 (48 kDa, lane 1) and myc-PRL-3 (21 kDa, lane 2), but did not cross-react with myc-PRL-1 and myc-PRL-2 (lanes 3 and 4). Lower panel showed the equal loading of myc-PRL-3, -1, and -2 (21 kDa).