Figure 1.

Growth inhibition induced by the PI3K inhibitors, NVP-BEZ235 or GDC-0941, and the MEK inhibitors, AZD6244 or PD0325901, alone and in combination. HCT116 and HT29 cells were treated with the indicated fractions of the GI₅₀ concentrations of the inhibitors, alone or in combination, derived from Supplementary Figure S1, for 72 h, and an SRB assay was subsequently performed. Data are presented as a percentage of the control, in which cells were treated with 0.5% (v/v) DMSO. Points represent the mean of three independent experiments±standard error. Lines were fitted using non-linear regression analysis.