Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Mar 27;106(8):1446–1452. doi: 10.1038/bjc.2012.95

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2012 Cancer Research UK

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Analysis of NT5E expression and methylation in malignant melanoma. (A) Expression of NT5E correlates inversely with CpG methylation in melanoma cell lines. The upper panels are RT–PCR analysis of NT5E and the control gene GAPDH in melanoma cell lines as indicated. Lower panels are MSP analysis of the NT5E CpG island in the same cell lines. There is complete methylation in Mel501 and partial methylation in Mel505. (B) Pyrosequencing analysis of the NT5E CpG island in a panel of melanoma cell lines. Pyrosequencing was done as described in Materials and Methods. The level of methylation is represented by the intensity of shading in the circles, each of which represents an individual CpG dinucleotide in the amplified fragment. The mean % CpG methylation in the amplified fragment is also shown. (C) qPCR analysis of NT5E mRNA levels in panel of melanoma cell lines. Data shown are means of at least three duplicates±1 s.d.