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. 2012 Mar 27;106(8):1446–1452. doi: 10.1038/bjc.2012.95

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Copyright © 2012 Cancer Research UK

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Pyrosequencing analysis of the NT5E CpG island in WM35 and SKMel173 cell lines. The figure shows representative pyrograms. The shaded C residue is the control for bisulphite conversion: there must be no peak at this residue (non CpG cytosine/guanine), confirming 0% cytosine incorporation. % Methylation at each CpG is indicated.