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. 2012 Mar 27;106(8):1446–1452. doi: 10.1038/bjc.2012.95

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Copyright © 2012 Cancer Research UK

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Methylation in the NT5E CpG island in primary malignant melanoma. Pyrosequencing analysis of the NT5E CpG island in primary malignant melanomas from sun-exposed sites was done as described in Materials and Methods. The level of methylation is represented by the intensity of shading in the circles, each of which represents an individual CpG dinucleotide in the amplified fragment. The mean % CpG methylation in the amplified fragment is also shown. The anatomical site of the primary lesion is indicated, together with the Breslow thickness (BT) in mm and the melanoma type (where available): NMM=nodular malignant melanoma; MM=malignant melanoma, sub-type not available; SSMM=superficial spreading malignant melanoma. Site(s) of metastasis (if applicable) are indicated: Br=brain; Cu=cutaneous; Li=liver; LN=lymph Node; Lu=lung; Or=orbit; Pa=parotid gland; Sp=spleen.