Skip to main content
. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Am J Transplant. 2011 Nov 9;12(3):590–599. doi: 10.1111/j.1600-6143.2011.03847.x

Figure 6. iNKT cells activation by conventional T cell alloresponses promotes effector cytokine release.

Figure 6

Supernatants from TEa T cells and iNKT cells cultured alone, or with irradiated stimulators (BALB/cxB6F1) or together, were obtained at 24, 48 and 72 hours and the presence of IFNγ determined by cytokine bead array (A). TEa T cells and iNKT cells were purified by cell sorting. MLR were carried out with iNKT cells cultured in a titration with TEa T cells (1:1-1:16) with stimulators and IFNγ measured in supernatants by ELISA on day 4 (B). iNKT cells were cell sorted and cultured in the presence TEa supernatant and anti-CD25 or isotype mAb and IL-2 and IFNγ measured by CBA (C). Data is presented as the mean of triplicate cultures ± s.d. of one experiment. *p=<0.05. Data is representative of 2-3 independent experiments.