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(–)Sch B-induced reactive oxygen species (ROS) production in H9c2 cardiomyocytes. (–)Sch B (7.5 or 15 μM) was incubated with H9c2 cardiomyocytes. Vehicle (i.e., DMSO) was added in untreated cells. Fluorescence intensity was monitored every minute for 360 minutes, as described in Materials and methods. Data, which were normalized with time-matched control, were expressed as a percentage of value at time zero. Values given are the mean of triplicate data
