Table 1.
Summary of binding and competition data.
3xmutTLC1-KBS RNA | TLC1-KBS RNA | Telomeric 3'overhang | Random 3'overhang | Blunt end | |
---|---|---|---|---|---|
Apparent Kd [nM] | 353 ± 49 | 10.5 ± 0.7 | 0.17 ± 0.10 | 0.30 ± 0.22 | 0.18 ± 0.06 |
koff [min−1] | 2.6 ± 1.2 | 0.005 ± 0.001 | 0.011 ± 0.002 | 0.007 ± 0.005 | |
t1/2 [min] | 0.27 | 140 | 63 | 99 | |
kon [M−1S−1] | 3.4×106 | 4.8×105 | 5.9×105 | 6.3×105 | |
Ki for TLC1-KBS RNA [nM] | 51 ± 32 | 13 ± 5 | 19.5 ± 8.3 | ||
IC50 for DNA [nM] | 40 ± 3 | 42 ± 9 | 38 ± 6 |