Fig. 2.

mRNA-Seq analysis: CR decreases igfn1 expression. Shown is a UCSC browser view illustrating the igfn1 (Immunoglobulin-like and fibronectin type III domain containing 1) gene with sequence coverage depth (quantity of reads, y-axis) at the top (blue, control; red, CR). The peaks mark expressed exons and are consistent with RefSeq annotation (dark blue at bottom). Decreased expression of igfn1 in CR muscle is indicated by the considerably fewer reads mapping to the exons in CR compared with control. Splice junctions identified by Cufflinks from control and CR datasets are shown below the sequence alignments. The igfn1 transcripts assembled by Cufflinks independently of any gene annotation are shown in green (isoform-a and isoform-b); the isoforms are the products of alternative splicing and both have alternative last exons.