Fig. 5.

A novel isoform of the Slc37a4 gene mRNA, featuring an exon not present in the annotated transcript databases. Screenshots from the UCSC genome browser, displaying the mRNA-Seq reads from control (blue) and CR muscle (red) samples mapping to annotated and novel exons in the Slc37a4 gene locus. Novel splice junctions detected by Cufflinks are also depicted. The stack of sequence reads in the middle identifies a novel exon present in both CR and control muscle samples (novel transcript in green). UCSC genome browser tracks for mainstream annotated transcript databases are shown, with no coding sequences annotated in the new exon region. The mammalian conservation track is at the bottom, showing conservation of the novel exon. Arrows beneath the exons flanking the novel exon represent position of forward and reverse PCR primers.