Fig. 6.

A novel isoform of Lmo7 mRNA. Screenshots from the UCSC genome browser, displaying mRNA-Seq reads from control and CR muscle samples mapping to annotated and novel exons in the Lmo7 gene locus. The reads aligned to the Lmo7 locus are shown at top (control, blue; CR, red). The novel junctions detected by splice junction reads are also depicted. Lmo7 transcripts annotated in the mainstream transcript databases are shown in the lower portion of the figure. The middle stacks of sequence reads identify the novel exon in both CR and control muscle samples, which is conserved as indicated by the mammalian conservation track (bottom). Arrows beneath the first and second exons of the novel transcript represent positions of the forward and reverse PCR primers used to verify the isoform.