Fig. 9.

Isoforms of Dcaf8 mRNA change with CR. Screenshots from the UCSC genome browser, displaying mRNA-Seq reads from control and CR muscle samples mapping to the Dcaf8 gene locus, with insets enlarging 2 regions. Reads aligned to the Dcaf8 locus are shown at top (control, blue; CR, red). The canonical RefSeq Dcaf8 mRNA is at bottom. Dcaf8 mRNA isoforms assembled by Cufflinks are in green. Isoform-b has the same transcription start site as -a, but a longer leader exon and an alternative 3′ end that drops the last 8 exons. Isoform-c has an alternative TSS. Isoform-d is a novel isoform initiated upstream of the 7th RefSeq canonical exon and thus lacking the first 6 exons. Arrows beneath exons of the novel transcripts represent positions of the forward and reverse PCR primers used to verify the isoforms as shown in Fig. 3G.