Table 1.

Cytokine profile of cells primed on OVA or APLs in the presence and absence of TGFβ

Priming peptide	± TGFβ1	Triple	Double	IL-2+, IFN-γ+	IL-2+, TNF-α+	IFN-γ+, TNF-α+	Single	IL-2+	IFN-γ+	TNF-α+	No cytokines	Cumulative
												IL-2+	IFN-γ+	TNF-α+
OVA	−	33.98	29.29	27.02	2.27	0.00	26.58	26.34	0.18	0.06	10.24	89.61	36.31	61.18
	+	35.28	28.59	24.12	4.35	0.13	24.51	24.25	0.26	0.00	11.60	88.00	39.76	59.79
Y3	−	46.99	21.14	17.91	3.14	0.09	19.73	19.46	0.27	0.00	12.10	87.50	50.22	65.26
	+	36.38	17.63	12.92	4.47	0.24	22.01	21.83	0.12	0.06	24.05	75.60	41.15	49.66
Q4	−	51.03	14.45	11.97	2.48	0.00	20.74	20.52	0.18	0.05	13.76	86.00	53.56	63.18
	+	35.66	14.11	9.94	3.90	0.26	19.00	18.80	0.17	0.03	31.27	68.30	39.86	46.03
T4	−	59.10	10.52	6.17	4.07	0.28	12.45	12.03	0.28	0.14	17.86	81.37	63.59	65.83
	+	33.56	9.94	5.24	4.38	0.32	14.22	13.72	0.40	0.11	42.27	56.90	38.37	39.52
V4	−	67.47	7.26	3.93	2.62	0.71	5.95	5.55	0.20	0.20	19.31	79.58	71.01	72.32
	+	24.67	8.19	2.54	5.22	0.44	17.69	17.58	0.07	0.04	49.45	50.01	30.37	27.72

OT-I splenocytes were primed and restimulated as shown in Fig. 1. The percentages of the resulting effector CD8⁺ T cells producing a particular set of cytokines or all of those producing an individual cytokine (Cumulative) are shown. Data shown are representative of at least three individual experiments with similar results