Figure 7.
Expression of STY1 is reduced in the drnl-2 and drn-1 drnl-1 puchi-1 mutant backgrounds. A, qRT-PCR-detected expression of STY1 was reduced at 10 d after germination in seedlings of drnl-2 and drn-1 drnl-1 puchi-1 mutant lines. Shown are averages of three biological replicates (three technical replicates per biological sample). Error bars represent se. The asterisk for drnl-2 denotes a significant reduction in STY1 expression compared with that of Ler (Student’s t test, P < 0.05); the asterisk for drn-1 drnl-1 puchi-1 denotes a significantly reduced expression level compared with both Ler and Col (Student’s t test, P < 0.05). Both ecotypes were used for comparison, as drn-1 and puchi-1 are in the Col background, whereas drnl-1 is in the Ler ecotype. B to I, STY1pro:GUS expression is reduced in drnl-2 seedlings and flowers. B and C, GUS staining (2 h of incubation) is reduced in cotyledons of 1-d-old drnl-2 seedlings compared with wild-type (wt) seedlings. D and E, No GUS staining was detected in cotyledons and the shoot apex (arrowheads) of 3-d-old drnl-2 seedlings after 2 h of incubation, whereas STYpro:GUS expression was strong at these sites in wild-type seedlings. F to I, STYpro:GUS activity was strongly reduced in drnl-2 ovules (arrows) compared with the wild type both after 6 h (F and G) and 24 h (H and I) of incubation.