Table III. Quantitative real-time RT-PCR analysis of PAL, CHS, and CAD gene expression in leaves of rose from plants either treated with (Si+) or without (Si−) Si followed by either inoculation with P. pannosa or no inoculation.

Values shown represent fold up- or down-regulation in Si− inoculated, Si+ uninoculated, and Si+ inoculated plants relative to Si− uninoculated plants (relative expression ratio = 1) at each time point, after normalization of all treatments to 18S rRNA. Data represent results from one experiment, and each observation represents the mean from three extractions. All values are presented as means ± se. The findings of this experiment were confirmed in a second independent experiment. * Significant change; ns, nonsignificant change.

Genes	Si Supply	Pathogen	Fold Change
			0 hai	24 hai	72 hai	120 hai
PAL	Si−	Uninoculated	1.0	1.0	1.0	1.0
	Si−	Inoculated	−1.7 ± 0.17ns	3.7 ± 1.25*	1.5 ± 0.17ns	1.1 ± 0.40ns
	Si+	Uninoculated	−1.6 ± 0.14*	2.6 ± 0.60*	1.2 ± 0.27ns	−1.0 ± 0.25ns
	Si+	Inoculated	−1.4 ± 0.11*	5.2 ± 1.49*	39.3 ± 11.6*	3.0 ± 0.58*
CHS	Si−	Uninoculated	1.0	1.0	1.0	1.0
	Si−	Inoculated	1.3 ± 0.46ns	2.8 ± 1.06*	1.5 ± 0.30*	1.2 ± 0.14ns
	Si+	Uninoculated	−1.4 ± 0.24*	2.6 ± 0.55*	2.6 ± 0.30*	2.2 ± 0.15*
	Si+	Inoculated	−1.0 ± 0.32ns	4.5 ± 0.45*	2.7 ± 0.38*	2.1 ± 0.36*
CAD	Si−	Uninoculated	1.0	1.0	1.0	1.0
	Si−	Inoculated	1.3 ± 0.21*	1.6 ± 0.59ns	−1.8 ± 0.04ns	−1.2 ± 0.23*
	Si+	Uninoculated	2.1 ± 0.40*	−0.3 ± 0.26ns	−1.1 ± 0.12ns	1.1 ± 0.39ns
	Si+	Inoculated	1.2 ± 0.28*	2.0 ± 0.17*	2.4 ± 0.55*	2.5 ± 0.40ns