Expression of SA-related genes in response to P. brassicae at 24 hpi. Plant leaves were agar plug inoculated with 8-d-old mycelium. Mycelium-free plugs were used for mock treatment. The transcript accumulation of ICS1, PR-1, and NPR1 was analyzed by real-time qPCR. After normalization with UBQ10, results were expressed as fold change in transcript level compared with the time zero sample (mean ± se; n = 3) from three independent biological experiments. Hash marks indicate statistically significant differences between mock and infected samples (P < 0.05), and asterisks indicate statistically significant differences between infected Col-0 and infected pad2-1 samples (P < 0.05), using unpaired heteroscedastic Student’s t test.