Figure 4.

Secondary structure comparison among human, yeast, and Arabidopsis mediator sequences. The protein secondary structures were predicted using PSIPRED and superimposed on the alignments generated using MAFFT. The purple rectangles and green arrowheads denote the predicted protein helices and sheets, respectively. A solid black line indicates no secondary structure, and a dotted line denotes a gap in the alignment. Each red bar represents a length equivalent to 20 amino acids. Blue lines indicate the extent of interaction of Med4 with Med7 and Med21. Hs, Human; Sc, S. cerevisiae; At, Arabidopsis.