Figure 7.

The positive role of AtAIRP2 in ABA induction of drought stress-related gene expression required SnRK protein kinase activity. A, ABA induction profiles of AtAIRP2 in wild-type (WT), abi1-1, snrk2.2, snrk2.3, and snrk2.6 single knockout mutant, and snrk2.2 snrk2.3 snrk2.6 triple mutant plants. Light-grown, 10-d-old wild-type and various snkr2 mutant seedlings were treated with 100 μm ABA. Total RNA was extracted from the treated tissues and analyzed by real-time qRT-PCR. RAB18 was a positive control for ABA induction, and UBC10 was used as a loading control. B, ABA induction profiles of drought-related genes in wild-type, atairp2-2, and AtAIRP2-overexpressing plants. Light-grown, 3-week-old plants were incubated with 100 μm ABA for 6 h. Induction patterns of various ABA- and drought-responsive genes (ABI1, ABI2, ABF3, ABF4, RD26, RD20, KIN2, and RAB18) were analyzed by real-time qRT-PCR. Data represent the fold induction of each gene by ABA (100 μm) relative to the control treatment (0 μm ABA). Mean values from three independent technical replicates were normalized to the levels of an internal control, glyceraldehyde-3-phosphate dehydrogenase C subunit mRNA. [See online article for color version of this figure.]