Figure 4.
A, Quantification of root-expressed type-A RRs. Relative expression levels in medicago pooled control roots (empty vector) versus roots harboring the MtRR9-RNAi construct. Quantification was normalized using stably expressed reference genes MtGAPDH and MtPTB. Bars, sd of three technical repeats. B and C, Number of emerged lateral roots (B) and lateral root primordia (C) per transgenic root of medicago plants harboring either a control (empty vector) or the MtRR9-RNAi construct. An asterisk indicates that the difference in number of emerged lateral roots between control and MtRR9-RNAi is statistically significant (Mann-Whitney U test, P < 0.05). Error bars, se (n = 46). D, Number of nodules per transgenic root of medicago plants harboring either a control (empty vector) or the MtRR9-RNAi construct. An asterisk indicates that the difference in nodule number between control and MtRR9-RNAi is statistically significant (Mann-Whitney U test, P < 0.05). Error bars, se (n = 37). Two independent biological replicates were performed for all experiments (A–d).