Figure 3.

In vivo interaction of AtDBP1 and AtDIP2. A, AtDBP1 interacts with AtDIP2 in the yeast two-hybrid system. Serial dilutions of yeast liquid cultures expressing the indicated constructs were plated on medium containing His (his+) and medium lacking His (his−) supplemented with 5 mm 3-amino-triazole (3AT). B, Top panels, confocal microscopy images showing the subcellular localization of AtDIP2 and AtDBP1 when fused to GFP and transiently expressed in leaves of N. benthamiana. GFP alone was included as a control. Bottom panels, BiFC assay. AtDBP1 and AtDIP2 were fused to N-terminal and C-terminal fragments of YFP and transiently expressed in N. benthamiana leaves by agroinfiltration. Left panel, confocal microscopy of leaves expressing YFPNt-AtDIP2 and YFPCt-AtDBP1; right panel, negative control consisting of AtDBP1 separately fused to both fragments. [See online article for color version of this figure.]