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. 2011 Sep 29;157(4):2056–2068. doi: 10.1104/pp.111.185199

Figure 3.

Figure 3.

In planta interactions of mutagenized ROXY1 with TGA proteins and subcellular localization of ROXY1 variants. A, Wild-type ROXY1 and the truncated form of ROXY1 lacking the C-terminal four amino acids (ROXY1Δ4) interact with TGA3 in the nucleus. The same nuclear interaction was also observed for PAN. B, All investigated ROXY1 substitution variants (ROXY1L1A, ROXY1L2A, ROXY1L3A, ROXY1L2/3A, and ROXY1L1/2/3A) failed to interact in planta, which is shown exemplarily for ROXY1L1A. C, Nucleocytoplasmic distribution of the GFP/ROXY1 fusion protein. D, Replacement of the first Leu (L1) with an Ala in the ROXY1 L**LL and removal of the ROXY1 C-terminal four amino acids showed a wild-type-like nucleocytoplasmic localization. All other mutagenized ROXY1 proteins also accumulated in the nucleus and the cytoplasm. Bars = 50 μm.