Figure 6.

SHM1pro-driven expression of SHM2 fused to the SHM1 presequence complements the shm1-1 allele. A, Schematic structure of the SHM1:SHM2 (S1/2) chimerical transgene. B, Eight-week-old wild-type (WT), shm1-1, and shm2-2 plants and two stably complemented shm1-1 mutant lines P10 and P20 transformed with the S1/2 chimerical transgene. Plants were grown for 6 weeks in air enriched with 0.15% CO₂ and then in normal air for another 2 weeks to allow direct comparison of growth with the shm1-1 mutant. C, Example of genotype verification in the transgenic lines, showing that line P20 is homozygous for the shm1-1 allele and harbors the SHM1:SHM2 chimerical transgene. [See online article for color version of this figure.]