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. 2011 Oct 4;157(4):2154–2166. doi: 10.1104/pp.111.183285

Figure 2.

Figure 2.

CHX treatment reduced FIT protein abundance and FIT gene expression in wild-type plants. A, FIT protein in roots of the wild type treated for 1 h with 50 μm CHX or without (control). Plants were grown in the hydroponic system at +Fe or –Fe, and samples were harvested directly after the treatment (0-h time point) and 4 h after retransfer to +Fe or –Fe growth medium without CHX (4-h time point). FIT protein was detected by western blot using anti-FIT-C polyclonal antiserum (top panel); Coomassie blue staining represents the loading control (bottom panel). B to D, Reverse transcription-quantitative PCR analysis of FIT (B), FRO2 (C), and IRT1 (D) in roots at +Fe and –Fe. E, Fe reductase activity of wild-type plants (Col-0). * Significant change (P < 0.05) versus +Fe; + significant change (P < 0.05) versus the non-CHX control; # significant change (P < 0.05) of the 4-h time point versus the 0-h time point. n = 2 (B–D) and n = 5 (E). [See online article for color version of this figure.]