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. 2011 Oct 4;157(4):2154–2166. doi: 10.1104/pp.111.183285

Figure 4.

Figure 4.

NO inhibition caused a reduction of FIT and HA-FIT protein levels. A, FIT protein in roots of the wild-type untreated (control) and plants treated for 24 h with 1 mm cPTIO. Plants were grown in the 6-d agar system at +Fe and –Fe. FIT protein was detected by western blot using anti-FIT-C polyclonal antiserum (top panel); Coomassie blue staining represents the loading control (bottom panel). B, HA-FIT in roots of HA-FIT 9 plants, treated and grown as in A. WT, Wild type. C, HA-FIT in roots of HA-FIT 9 plants, treated with 10 μm AVG and grown as in A. D, HA-FIT protein abundance in roots of –Fe HA-FIT 9 plants, untreated (control [contr]) or treated with 1 mm tungstate (Tung), 1 mm l-NAME (L-N), and 1 mm cPTIO, showing that several NO inhibitors caused reduction of HA-FIT protein amounts. Plants were grown as in A. In B to D, HA-FIT protein was detected by western blot using anti-HA monoclonal antibodies (top panels). In B and C, Coomassie blue staining represents the loading control (bottom panels); in D, Ponceau S was used as the loading control (bottom panel). [See online article for color version of this figure.]