Figure 6.

Loss of interaction activity with TaGID1 results in GA insensitivity. A, Morphologies of 10-d-old Rht-B1a, Rht-B1b, and Rht-B1c wheat seedlings treated with 50 μL L⁻¹ GA₃ (right) or without GA₃ (left). Bar = 5 cm. B, Comparison of seedling lengths of 10-d-old Rht-B1a, Rht-B1b, and Rht-B1c plants treated with (gray bars) or without (black bars) 50 μL L⁻¹ GA₃. Data were measured as means of 30 seedlings at each time with three replicates. Significance is as follows: * P ≤ 0.05, ** P ≤ 0.01. C, Relative variances of Rht-B1a, Rht-B1b, and Rht-B1c seedlings. The formula for variance is as follows: relative variance = (length of seedling with GA₃ − length of seedling without GA₃)/length of seedling without GA₃ × 100%. Significance is as follows: ** P ≤ 0.01. D and E, GA-mediated induction of α-amylase activity in Rht-B1a, Rht-B1b, and Rht-B1c seeds. A concentration of 1 μm GA₃ was applied to the plates. Sector I, Rht-B1a; sector ІІII, Rht-B1b; sector ІІІIII, Rht-B1c. F, Interaction between TaGID1 and Rht-B1. Y2H assays were performed using TaGID1 as bait and Rht-B1a, Rht-B1b, and Rht-B1c as prey. Left, growth of yeast strain AH109 transformants on −His−Ade plates; right, β-galactosidase activity detected in liquid assay with yeast strain AH109 transformants. To measure relative lacZ activity, three independent experiments were carried out. Five individual transformants were used each time. [See online article for color version of this figure.]