Figure 1.

spy mutants were more tolerant of high-salinity stress compared with wild-type plants. A, Morphological phenotypes of spy mutants. Three-week-old Col, spy-1, and spy-3 plants growing on GM agar plates were photographed. Ler, Ecotype Landsberg erecta. B, Schematic structure of the SPY protein and the locations of the mutations in the mutants. C, spy-1, spy-3, and wild-type (WT) plants grown on GM plates for 7 d and subsequently transferred onto 0.5× MS medium plates with or without 200 mm NaCl. D, The survival rate was calculated from independent experiments (n ≥ 3; ** P < 0.01, * P < 0.05, Student’s t test). E, Salt stress tolerance was compared between 8-d-old Col and 7-d-old spy-3 plants, as described in A.