Figure 6.

Overexpression of SPY reduced plant drought stress tolerance. A, RNA gel-blot analysis of transgene expression in vector and 35S:SPY-transformed plants; 10 μg of total RNA was loaded. B, Morphological phenotype of 3-week-old SPY-OX plants as compared with the wild type. C, Survival rate of vector-transformed and SPY-OX lines. The averaged data were obtained from three independent experiments (** P < 0.01, * P < 0.05, Student’s t test). D, qRT-PCR analysis of the expression of genes that were up-regulated in spy-3 mutants but down-regulated in SPY-OX plants. White bars indicate the relative expression level in the untreated plants, and black bars indicate the relative expression level in the 2-h-dehydrated plants. Primer sequences are listed in Supplemental Table S11.