Figure 5.

Potential F. cylindrus DMSP pathway enzymes were screened for signal and transit peptides and variants of the diatom chloroplast-targeting motif ASAFAP. N-terminal variants are noted as alt for alternative splice variant. Asterisk (*) indicates significant probability of chloroplast-targeting presequence based on Target P and Signal P predictions. Bold letters, predicted signal peptide; gray highlight, predicted cleavage site motif; yellow highlight, manually predicted cleavage site based on motif characterization by Gruber et al. (2007); underlined, predicted transit peptide; red letters, conserved domain of mature protein.