Table I. Hypersalinity response proteins in F. cylindrus.
2DE proteins spots (SSP) are listed from highest to lowest relative fold-change calculated as a ratio of normalized mean spot intensity in 70 compared to 35 salinity cultures. P values are reported for each spot, along with JGI protein accession number, name, and KOG information. Table abbreviations include: pr., Precursor; chlor, chloroplast; KOG groups include: M, metabolism; C, cellular processes and signaling; I, information storage and processing; P, poorly characterized; NA, no KOG homolog.
| SSP | Fold Δ | P Value | Accession No. | Protein Name | KOG Group: Class |
| 7426 | 4.79 | 0.000 | 277974 | Acetylornithine aminotransferase | M: amino acid transport and metabolism |
| 270566 | Pyrophosphate-dependent phosphofructo-1-kinase | M: carbohydrate transport and metabolism | |||
| 5515 | 3.51 | 0.000 | 269005 | Orn aminotransferase | M: amino acid transport and metabolism |
| 4707 | 3.24 | 0.000 | 212856a | Putative sarcosine-dimethylglycine methyltransferaseb | P: general function prediction only |
| 6415 | 3.16 | 0.000 | 277974 | Acetylornithine aminotransferase | M: amino acid transport and metabolism |
| 185436 | Hypothetical protein | NA | |||
| 1312 | 3.15 | 0.005 | 273578 | Mitochondrial translation elongation factor EF-Tsmt | I: translation, ribosomal structure and biogenesis |
| 5404 | 3.13 | 0.000 | 203522 | Pyrroline-5-carboxylate reductase | M: amino acid transport and metabolism |
| 5511 | 3.10 | 0.006 | 269005 | Orn aminotransferase | M: amino acid transport and metabolism |
| 5505 | 2.76 | 0.005 | 269005 | Orn aminotransferase | M: amino acid transport and metabolism |
| 3518 | 2.75 | 0.018 | 207357 | Putative SAM-dependent carboxyl methyltransferaseb | NA |
| 204 | 2.70 | 0.003 | 261222 | Collagens (type IV and type XIII), and related proteins | C: extracellular structures |
| 2318 | 2.62 | 0.001 | 275170 | N-acetyl-gamma-glutamyl-phosphate reductase | M: amino acid transport and metabolism |
| 6517 | 2.51 | 0.000 | 271944 | S-adenosylmethionine synthetase | M: coenzyme transport and metabolism |
| 5701 | 2.41 | 0.007 | 177646 | ALDH | M: energy production and conversion |
| 3411 | 2.40 | 0.002 | 187807 | Glutamate 1-semialdehyde 2,1-aminomutase | M: amino acid transport and metabolism |
| 2616 | 2.37 | 0.001 | 238865 | Putative pyridoxal-dependent decarboxylaseb | NA |
| 4712 | 2.30 | 0.000 | 212856a | Putative sarcosine-dimethylglycine methyltransferaseb | P: general function prediction only |
| 403 | 2.26 | 0.003 | 212738 | Ser carboxypeptidase (lysosomal cathepsin A) | C: posttranslational modification, protein turnover |
| 4514 | 2.13 | 0.010 | 170146 | S-adenosylhomocysteine hydrolase | M: coenzyme transport and metabolism |
| 4510 | 2.11 | 0.001 | 170146 | S-adenosylhomocysteine hydrolase | M: coenzyme transport and metabolism |
| 7645 | 1.88 | 0.000 | 271944 | S-adenosylmethionine synthetase | M: coenzyme transport and metabolism |
| 5504 | 1.76 | 0.004 | 170146 | S-adenosylhomocysteine hydrolase | M: coenzyme transport and metabolism |
| 2105 | 1.68 | 0.000 | 269315 | ThiJ/PfpI family (putative transcriptional regulator DJ-1) | C: defense mechanisms; P: general function |
| 2606 | 1.65 | 0.019 | 263016a | DiDECARB | M: amino acid transport and metabolism |
| 4206 | 1.64 | 0.005 | 267582 | Glutathione S-transferase | C: posttranslational modification, protein turnover |
| 609 | 1.62 | 0.009 | 272749 | Iron starvation induced protein (ISIP2A)b | NA |
| 7429 | 1.58 | 0.005 | 268209 | Alcohol dehydrogenase class V | M: secondary metabolites biosynthesis, transport |
| 3413 | 1.55 | 0.004 | 270038 | Fructose 1,6-bisphosphate aldolase | M: carbohydrate transport and metabolism |
| 5201 | 1.51 | 0.010 | 267582 | Glutathione S-transferase | C: posttranslational modification, protein turnover, chaperones |
| 7205 | 1.50 | 0.003 | 173405 | NADPH-dependent FMN reductase (predicted flavoprotein) | P: general function prediction only |
| 3315 | 1.41 | 0.004 | 170890 | Fructose- 1,6-bisphosphatase | M: carbohydrate transport and metabolism |
| 4407 | 1.40 | 0.020 | 187808 | Glu 1-semialdehyde 2,1-aminomutase | M: amino acid transport and metabolism |
| 1513 | 1.37 | 0.017 | 273803 | Unknown transaminase (kynurenine aminotransferase) | M: amino acid transport and metabolism |
| 1011 | 1.31 | 0.000 | 269494 | Cu2+/Zn2+ SOD1 | M: inorganic ion transport and metabolism |
| 6422 | 1.26 | 0.011 | 258994 | Fructose 1,6-bisphosphate aldolase | M: carbohydrate transport and metabolism |
| 5312 | 1.22 | 0.013 | 269867 | Glyceraldehyde-3-phosphate dehydrogenase | M: carbohydrate transport and metabolism |
| 207931 | Transaldolase | M: carbohydrate transport and metabolism | |||
| 8407 | 1.19 | 0.002 | 268607 | Pyridoxal (vitB6) biosynthesis lyase (stationary phase-induced protein) | M: coenzyme transport and metabolism |
| 6310 | −1.35 | 0.005 | 272954 | Hypothetical protein | NA |
| 213 | −1.43 | 0.009 | 203554 | DNA polymerase Δ processivity factor (PCNA) | I: replication, recombination, and repair |
| 10 | −1.45 | 0.019 | 272028 | Fucoxanthin-chlorophyll a-c binding protein, chlor pr. | NA |
| 23 | −1.49 | 0.016 | 272028 | Fucoxanthin-chlorophyll a-c binding protein, chlor pr. | NA |
| 2018 | −1.52 | 0.001 | 272228 | Cytochrome b6-f complex iron-sulfur subunit, chlor | M: energy production and conversion |
| 2012 | −1.53 | 0.001 | 269837 | ATP-dependent Clp protease adaptor protein ClpS | NA |
| 170761 | Fucoxanthin-chlorophyll a-c binding protein C, chlor pr. | NA | |||
| 3008 | −1.54 | 0.011 | 273006 | Fucoxanthin, chlorophyll protein1 | NA |
| 2106 | −1.76 | 0.018 | 174589 | Fucoxanthin chlorophyll a/c-binding protein pr. | NA |
| 4105 | −1.90 | 0.007 | 206118 | PSII oxygen evolving complex protein | NA |
| 5116 | −1.92 | 0.004 | 172948 | Chloroplast light harvesting protein isoform12 | NA |
| 221 | −2.01 | 0.016 | 268130 | FKBP-type peptidyl-prolyl cis-trans isomerase | C: posttranslational modification, protein turnover |
| 3003 | −2.04 | 0.007 | 170761 | Fucoxanthin-chlorophyll a-c binding protein D, chlor pr. | NA |
| 309 | −2.31 | 0.015 | 261222 | Collagens (type IV and type XIII), and related proteins | C: extracellular structures |
| 1105 | −2.62 | 0.002 | 243823 | Hypothetical protein | NA |
| 2008 | −2.63 | 0.002 | 170761 | Fucoxanthin-chlorophyll a-c binding protein C, chlor pr. | NA |
| 2107 | −2.68 | 0.011 | 174584 | Fucoxanthin chlorophyll a/c-binding protein pr. | NA |
a
Protein accession from all models database.
b
Protein name manually annotated based on National Center for Biotechnology Information blastp search (see Supplemental Table S7).