Figure 9.

Drought responses of the root system in the expression of genes encoding the porphyrin enzymes related to heme metabolism. A, Common branch. B, Heme branch. The plants were subjected to the same treatments as in Figure 8. Cont1 and Cont2, Nondesiccated controls for mild and severe drought stress, respectively; Mild, mild drought stress under air drying for 4 h; Severe, severe drought stress under air drying for 8 h. Total RNAs were purified from plants and reverse transcribed. The resultant cDNAs were used as templates for qRT-PCR using Actin as an internal control. The wild-type (WT) control was used for normalization, with the expression level of the sample set to 1. Error bars represent se, and representative data from three independent experiments are presented.